EVALUATION OF PRELIMINARY ANTIBACTERIAL ACTIVITY AND UV-SPECROSCOPIC ANALYSIS OF MICHELIA CHAMPACA (L.)

T. Lavanya and T. Ananthi*

ABSTRACT

The aim of the present study was undertaken to appraise the UV-VIS analysis and antibacterial activity of flowers of Michelia champaca. The phytochemical analysis revealed the presence of carbohydrate, alkaloids, terpenoids, flavonoids, tannins, steroids and phenols. The UV-VIS profile of flower extract showed the peaks at 281.1 nm and 215.6 nm with the absorption of 2.397 and 4.040. The antibacterial activity of flower extract against gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative bacteria (E.coli) were tested by using disc diffusion method. Ampicilin was used as standard. The results obtained shows that the extract expressed remarkable antibacterial activity with zone of inhibition ranging from 9 to 19 mm. Maximum inhibition zone was recorded with Bacillus subtilis (19.6 mm) while minimum inhibition zone was recorded for E.coli (9.37mm). The antibacterial activity increased linearly with increase in concentration of the extract. The antibacterial activity of Michelia champaca was due to the presence of various bioactive constituents present in the flower extract. The results provide a support for the use of this plant in traditional medicine and it is a potential antiseptic source for prevention and treatment of bacterial infections.

Keywords: Michelia champaca, UV-Visible, ampicilin, antibacterial activity.