RAPID, COST-EFFECTIVE AND ACCURATE QUANTIFICATION OF DIPHENYLMETHANE DERIVATIVES BISPHENOL A USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Dr. SK Manirul Haque*
High performance liquid chromatographic method with isocratic elution was developed for the determination of Bisphenol A in canned food and drinking water bottle samples. The method was validated according to international conference on harmonization guidelines. The BPA was separated using C18 Hypersil BDS column with flow rate 1.0 ml/min. The sample was detected at 254 nm applying photo diode array detector. The linearity of the proposed was in the range of 3.5 – 25.0 μg/ml. The LOD and LOQ value were found to be 0.76 and 2.53 μg/ml respectively. The method can be used for routine quality control samples as well as for research purposes for the determination of BPA.
Keywords: Bisphenol A; HPLC; Validation; Column; ICH guidelines; Canned food; Drinking water bottles.
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