EXPLORATION OF PRIMARY METABOLITES FROM LEMNA MINOR AND DETERMINED ITS IMMUNOMODULATORY AND ANTIMICROBIAL ACTIVITY

Vishakha S Mane, Amit Gupta*, Nilima Pendharkar and Bharat Shinde

ABSTRACT

Objective- The objective of our study is to examine its antimicrobial and immunomodulatory activity of aqueous extract of whole plant i.e. Lemna minor. Methods- In this study, we evaluated its protein and amino acid content in aqueous extract and also determining its antimicrobial activity on four bacterial strains and one fungal strain. In addition, Elisa experiment was performed in order to determined its immunomodulatory potential against specific protein antigen i.e. ovalbumin. Results- The results showed its enhancement in protein content (Nanodrop method) at a very low concentration of aqueous extract and also showed its rich content in amino acids. In addition, aqueous extract at higher doses showed enhancement in antibody titre against ovalbumin and decline in proliferation rate of these four bacterial and one fungal strains at higher doses as compared to control. Conclusion- Overall data indicates its immunomodulatory and antimicrobial activity of Lemna minor.

Keywords: Lemna minor; antimicrobial; immunomodulatory; bacteria; fungus.