EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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Abstract

BIOLOGICAL CONTROL OF WILT CAUSING PSEUDOMONAS SPP. BY THE USE OF LEAF EXTRACTS OF SCHIMA WALLICHII (DC.) KORTH. AND MELASTOMA MALABATHRICUM L.

Sunrit Basu Sarbadhikary and Narayan C. Mandal*

ABSTRACT

Vascular wilt is a common disease of solanaceous crops caused by many fungal and bacterial species. Two wilt symptoms producing bacterial strains VBLE3 and VBST13 were isolated from infected tomato plants. Both the strains were identified as Pseudomonas spp. by 16s rRNA gene sequence homology. The pathogenecity of the bacterial strains were also proved by reproduction of disease in pot experiments. Leaf extracts of two indigenous angiosperm species of Tripura, viz., Schima wallichii and Melastoma malabathricum showed very good antagonistic effects against both of the wilt pathogens. Prominent zones of inhibition against the pathogens were produced by both leaf extracts when tested by disc diffusion method in the range of 15 to 20 mm. Massive reduction in the numbers of colony forming units (CFU) of VBLE3 and VBST13 were also noticed when treated with these plant extracts. The Minimum Inhibitory Concentration (MIC) value of the crude extracts of Schima wallichii and Melastoma malabathricum was found to be 100 μg/mL and 150 μg/mL respectively for both the strains. Schima wallichii showed bactericidal and Melastoma malabathricum showed bacteriostatic mode of action against both the bacterial strains.

Keywords: Vascular wilt, pot experiment, leaf extracts, MIC, bactericidal, bacteriostatic.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia