EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

THEOBROMA CACAO STEM BARK AMELIORATES DOXORUBICIN-INDUCED OXIDATIVE STRESS, TESTICULAR AND LUNGS DAMAGE IN EXPERIMENTAL RATS

Kosoko A. M.*, Olurinde O. J. and Akinloye O. A.

ABSTRACT

Doxorubicin (DOX), a chemotherapy drug, is used to treat different types of cancer but its clinical use has been limited by dose-dependent multi-organ toxicities especially to the lungs, testes, heart and liver. Eighty rats, randomly selected, were divided into three (3) treatment groups: pre-, co- and post-treatment groups; consisting of 6 sub-groups each (5 rats per sub-group); baseline, normal saline (2ml), α- lipoic acid (20mg/kg body weight), and 200mg/kg, 400mg/kg or 800mg/kg body weight Theobroma cacao stem bark aqueous extract (TCAE) sub-groups. All animals except those in baseline sub-group were intoxicated with 20mg/kg body weight DOX intraperitoneally. Animals in the pre-treatment group were administered a single dose of DOX followed by 7 days oral administration of normal saline, α- lipoic acid or graded doses of TCAE; co-treatment group were co-administered 2.86 mg/kg body weight DOX with either normal saline, α- lipoic acid or TCAE orally for 7 days while post treatment group were administered normal saline, α- lipoic acid or TCAE orally for 7 days and on the 8th day, intoxicated with a single dose of DOX. Animals were sacrificed (pre- and post- treatment group were sacrificed on the 9th day while the co-treatment group sacrificed on the 8th day), blood samples collected by retro-orbital plexus, testes and lungs harvested for biochemical assays and histopathological investigations. Data were analyzed using SPSS 20.0 statistical tool, significance of difference calculated using one-way analysis of variance (ANOVA) and Duncan multiple range test. DOX intoxication caused a significant increase in testicular and lungs acid phosphatase, lactate dehydrogenase, γ-glutamyl transferase activities with a concomitant decrease in alkaline phosphatase activity. DOX intoxication also caused a significant increase in the concentrations of hydrogen peroxide, malondialdehyde and protein carbonyl; activities of myeloperoxidase, NADPH oxidase and xanthine oxidase while significantly reducing the concentrations of reduced glutathione, ascorbic acid and α-tocopherol; and activities of catalase, superoxide dismutase, glutathione-S-transferase and glutathione peroxidase. Treatment with TCAE significantly ameliorated markers of testicular and lungs damage and pro-oxidant markers while elevating the organs antioxidant status. TCAE possess a potential ameliorative property against DOX induced testicular and lungs damage and oxidative stress.

Keywords: Chemoprevention; Theobroma cacao; Doxorubicin; Testicular toxicity; Lungs toxicity; Oxidative stress.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )