HISTOPATHOLOGY ACTIVE BIPHENYL PYRROLE COMPOUND ISOLATED FROM MARINE BREVIBACTERIUM SPECIES

V. Srilekhaa, B. Prasanna*, A. Sridhar Rao, P. ShivaKrishna, V. Sheshasrinivas, M. A. Singaracharya*

ABSTRACT

A Biphenyl pyrrole (1) was identified in the marine Brevibacterium sp which was extracted from crude pigment and purified through RP-HPLC. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that the bacterium was closely related to the type strains of Brevibacterium species (99%). The bacterial strains which showed significant antimicrobial activity were identified to genus level by PCR amplification of the 16S rRNA gene, BLAST analysis, and comparison with sequences in the GenBank nucleotide database and the species level was identified as the nearest phylogenetic neighbor with sequences >99% sequence similarity. The Wound healing activity against Wistar Albino rats was also studied by using Safromycin ointment as standard drug. The complete structure of compound (1) was confirmed through IR, NMR and Mass spectral data.

Keywords: Brevibacterium sp, Marine bacteria, Biphenyl pyrrole, Wound healing, Wistar Albino.