DEVELOPMENT OF SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF AMIKACIN SULPHATE IN PURE AND PHARMACEUTICAL FORMULATIONS USING ASCORBIC ACID

Mohamed E. Adam, Shaza W. Shantier*, Malik A. Hussien, Elrasheid AE Garalnabi and Elrasheed A. Gadkariem

ABSTRACT

In this study, a simple spectrophotometric method was developed for the determination of amikacin sulphate (AK)
in pure bulk form and in its pharmaceutical formulations. Amikacin reacted with ascorbic acid to form a water
soluble, purple pink, 1:1 complex that showed two wavelengths maxima (λmax) at 390 nm and 530 nm. The color
was developed after heating for 40 minutes at 100 ËšC and remained stable for at least 48 hours. The validity of
Developed method was tested by analysing amikacin working standard under the optimum experimental
conditions. Beer’s lambert law was obeyed over the concentration range 40-200μg /ml with an excellent correlation
coefficient (not less than 0.999). The repeatability and reproducibility results showed a low relative standard
deviation values (RSD % < 2) at λmax 390nm and 530nm, which reflected the precision of the developed method.
The assay results for commercial amikacin injection (500mg/2ml) were (99.89 ± 0.75 %, n=4) and (99.9 ± 0.5 %,
n=4) at λmax 530 nm and 390 nm, respectively. The percentage added recovery was found to be about (100.76 ± 1.2
%, n=3) at both wavelength, which reflected the freedom from interferences.

Keywords: Amikacin, Spectrophotometric, Ascorbic acid.