EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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Abstract

DEVELOPMENT OF SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF AMIKACIN SULPHATE IN PURE AND PHARMACEUTICAL FORMULATIONS USING ASCORBIC ACID

Mohamed E. Adam, Shaza W. Shantier*, Malik A. Hussien, Elrasheid AE Garalnabi and Elrasheed A. Gadkariem

ABSTRACT

In this study, a simple spectrophotometric method was developed for the determination of amikacin sulphate (AK)
in pure bulk form and in its pharmaceutical formulations. Amikacin reacted with ascorbic acid to form a water
soluble, purple pink, 1:1 complex that showed two wavelengths maxima (λmax) at 390 nm and 530 nm. The color
was developed after heating for 40 minutes at 100 ËšC and remained stable for at least 48 hours. The validity of
Developed method was tested by analysing amikacin working standard under the optimum experimental
conditions. Beer’s lambert law was obeyed over the concentration range 40-200μg /ml with an excellent correlation
coefficient (not less than 0.999). The repeatability and reproducibility results showed a low relative standard
deviation values (RSD % < 2) at λmax 390nm and 530nm, which reflected the precision of the developed method.
The assay results for commercial amikacin injection (500mg/2ml) were (99.89 ± 0.75 %, n=4) and (99.9 ± 0.5 %,
n=4) at λmax 530 nm and 390 nm, respectively. The percentage added recovery was found to be about (100.76 ± 1.2
%, n=3) at both wavelength, which reflected the freedom from interferences.

Keywords: Amikacin, Spectrophotometric, Ascorbic acid.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia