EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

EVALUATION OF SEEPLEX PNEUMOBACTER MULTIPLEX PCR KIT FOR THE DETECTION OF COMMUNITY-ACQUIRED PNEUMONIA PATHOGENS AMONG OUT PATIENTS IN UNIVERSITY OF CALABAR TEACHING HOSPITAL, CALABAR

Edeh E.B., Alaribe A.A., Ogba O.M.*, Mandor B.I., Adat P.E., Nwaokorie F.O. and Obo U.U.

ABSTRACT

Objectives: Identification of bacterial etiologic agents promptly and correctly is crucial in the management of community-acquired pneumonia (CAP). This study evaluates the analytical working effectiveness and clinical value of a PCR assay in identifying bacterial pathogens using Seeplex Pneumobacter multiplex PCR detection kit. Materials and Methods: Sputa from 120 clinically confirmed CAP out-patients aged between 5 and 75 years were analyzed by conventional culture. Fifty specimens were randomly selected from all the age groups and analyzed by multiplex PCR for the detection of Streptococcus pneumoniae, Legionella pneumophila, Haemophilus influenzae, Chlamydophila pneumoniae, Bordetella pertussis and Mycoplasma pneumoniae. Results: Forty two specimens (35%) were positive culturally, with Moraxella catarrhalis 20 (47.6%) being the most isolated organism. S. pneumoniae was the most detected CAP agent via PCR. Of the 50 specimens analyzed by multiplex PCR, 25(50%) were positive and S. pneumomiae, 21(42%) was the most detected organism. Comparing the 50 specimens evaluated using both methods: twenty one S. pneumoniae and (14) H. influenzae were detected via multiplex-PCR while none of these two organisms was isolated via culture. Conclusions: Seeplex pneumobacter multiplex PCR kit could be of great value for detecting the etiologic agents of CAP quite promptly and accurately.

Keywords: Community- acquired pneumonia, Culture, Microscopy, Multiplex- polymerase chain reaction, Streptococcus pneumoniae.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )