EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

IDENTIFICATION OF CRYPTOSPORIDIUM SPP. INFECTIONS IN CHILDREN WITH PERSISTENT DIARRHEA BY MODIFIED ZIEHL-NEELSEN STAIN METHOD AND PCR TECHNIQUE.

Hashim Raheem Tairsh, Abdul AL-Karim Abdullah, Raad A. Al-A-asady, Mohamed J. AL-zeyadi, Hashim Ali Alsherees*, Adel N. M. AL-Baldawy

ABSTRACT

Background:Cryptosoridiosis is an intestinal infection caused by intracellular protozoan parasite Cryptosporidium, which may appear as a persistent diarrhea in children, that may lead to malnutrition, growth stunt and wasting. Since Cryptosporidium disease does not produce a specific clinical syndrome which allows to distinguish it from other intestinal infections; therefore, the final diagnosis requires a microscopic test for detection of the parasite in stool. Methods: Prevalence of cryptosporidiosis in children was studied in Najaf province by microscopic examination of stool samples by modiefied acid stain method in 140 children with persistent diarrhea, and the positive cases were confirmed by using SSU rRNA-based PCR-detecting technique. Results: The prevalence of cryptosporidiosis in children with persistent diarrhea was 12.85%, and the infection rate was higher in males (13.5%) than in females (12.1) and those with age less than 1 year (13.57%) than that of other age groups, and in children of the rural areas (14.3%) than that of the urban areas (10.5%). And the highest infection rate was recorded in May (3.57%). Conclusion: Cryptosporidium spp. is one of the most common enteric parasite that associate with persistent diarrhea in children.

Keywords: Cryptosporidiosis, Cryptosporidium, modified acid-fast stain, SSU rRNA gene.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia