EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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 ICV - 79.57

Abstract

PROXIMATE COMPOSITION, PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF DATURA STRAMONIUM L. LEAVES EXTRACT

Sami K. D. Idris and Shama I. Y. Adam*

ABSTRACT

Datura stramonium is used in the traditional medicine for a wide range of ailments. The aim of this work was to evaluate the proximate composition of D. stramonium leaves, to extract the leaves powder using solvents, petroleum ether, methanol and water and to identify the active constituent(s), and also to evaluate the Antimicrobial activity of these extracts. The Yield% of the leaves extracts using petroleum ether was 7.04%, methanol 7.78% and water was 19.05%. The proximate composition of plant leaves was assessed using the method described by the association of official analytical chemists and revealed 3.55% as moisture content, 15% ash and 84.45% for organic matter. The in vitro antimicrobial activity of 10 and 50% concentrations of petroleum ether, methanol and water extracts of D. stramonium against bacterial organism; Gram positive (Staphylococcus aureus) and Gram negative (Escherichia coli, Pseudomonas aeruginosa) and the fungus Candida albicans was carried out using the agar- well diffusion method. It was showon that the extracts have variable bactericidal activity. E.coli and Candida albican were the most sensitive organisms to different concentrations of extracts. The result also showed that the plant extracts contain: alkaloids, flavonoid, reducing compound, glycosides, saponins, and triterpens, but all were devoid of tannins. Thin Layer Chromatograpic technique (TLC) was used to separate active compounds of the leaves. The Gas chromatography-mass spectroscopy (GC/MS) analysis detected the presence of Scopolamine, Pyrazine, 1-Propanamine, Butanomic acid, Imidazole, Ascorbic acid and other types of constituents.

Keywords: Datura stramonium, antimicrobial activity, proximate composition, TLC, GC-MS techniques.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )