IN VITRO MICRO PROPAGATION AND TUBERIZATION OF EULOPHIA NUDA LIND AN ENDANGERED TERRESTRIAL ORCHID

Ruchi Kurapa Shroti and Dr. Ravi Upadhyay

ABSTRACT

Eulophia nuda lind commonly known as amarkand, malakand,belongs to family Orchidaceae is an important medicinal orchid so it posses position in Astraverga the known class of medicine in ayurvedic system of medicine due to its medicinal uses and beautiful flowers it is being Over exploited by local tribes, herbal health care, destruction of habitats by reclamation, loss of pollinators ,fragmentation of population is the fore most reason of it endangered stage (Brooks et al., 2002) (Appendix II of CITES), (Annex-2 PC-19 DOC.11.3-P-7) Orchids whose parts and derivatives traded mainly for Traditional Asian Medicine(TAM) or SALEP. It propagates at very low rate because seeds are very minute, non-endospermic and requires mycorrizhal association >0.1 % seeds germinates in nature the seeds so called ‘dust seeds (‘Fleischer 1929,Ziegenspech 1936.), therefore ,it is the high time to save of this potential source of medicine for sustainable utilization in mankind, so here we propose standardized protocol for invitro micro propagation of Eulophia nuda lind So here we described an efficient and reproducible protocol standardized for in vitro micro propagation of Eulophia nuda lind through axillary bud culture initiation induced in MS+3.0 (mg/l Bap),after 15 days of inoculation , shoot elongation in MS+3.0 mg/l BAP+1MG/L IAA, Spontaneous rooting obtained in MS +3MG/L BAP+1 MG/L IBA.

Keywords: Endangered orchid, medicinal, micropropaation.