ISOLATION, CHARACTERIZATION AND INVITRO ANTIDIABETIC ACTIVITY OF ?- GLUCAN ISOLATED FROM EDIBLE MUSHROOM PLEUROTUS FLORIDA.

S. Selva Kumar* and S. Shankar

ABSTRACT

Mushroom β-glucan has the ability to affect many cellular functions, including cellular glucose uptake. Although cumulative evidence in literature suggests a connection between β-glucan and reduction of blood glucose concentration, a mechanism of how β-glucan affects cellular glucose uptake has not been demonstrated. Isolation of glucans from raw sources needs removal of ballast compounds including proteins, lipids, polyphenols and other polysaccharides. Purity control of glucan fractions is necessary to evaluate the isolation and purification steps; more rigorous structural analyses of purified polysaccharides are required to clarify their structure. A set of spectroscopic, chemical and separation methods are used for this purpose. Among them, NMR spectroscopy is known as a powerful tool in structural analysis of glucans both in solution and in solid state. In this study, β glucan isoalated from Pleurotus florida ,characterized by 1H NMR, 13C NMR spectroscopy and the invitro antidiabetic effect of β-glucan by β-glucosidase inhibitor activity also analyzed. The highest inhibitory activity (76.68%) was detected at 2.0 mg/mL. This result indicated that β-glucan possessed higher inhibitory activitiy against α- glucosidase.

Keywords: ?-glucan, Pleurotus florida, invitro antidiabetic, ?-glucosidase inhibitor activity.