GC-MS ANALYSIS OF ETHYL ACETATE EXTRACT OF SEMECARPUS ANACARDIUM NUTS
*Rajendra Singh and Arun Kakkar
ABSTRACT
In present investigation phytochemical compounds of Semecarpus anacardium nuts using GC-MS technique was carried out to determine the structure of phytochemicals present in it. The mass spectra of the phytocompounds found was matched with the National Institute of Standards and Technology library. Dried SA nut was successively extracted with petroleum ether, ethyl acetate, methanol and water using
soxhlet apparatus. This extracts was purified and stored in refrigerator. The ethyl acetate extract of SA nuts was analyzed by Agilent 7890A GC with 5975MS. Nine compounds were indentified ie benzoic acid, 4-ethoxy ethyl ester, phenol. 2,4- bis (1,1-dimethyl), Eicosane, 7- hexyl. The compounds were identified by comparing their retention time and peak area with literature and structure determination was done by interpretation of mass spectra. Present investigation by GC-MS analysis of ethyl acetate extract of SA is done.
Abbreviation
SA = Semecarpus anacardium
GC-MS = Gas Chromatography Mass Spectroscopy
TLC = Thin layer Chromatography
NIST =National Institute Standard and Technology
TDA =Toluene-diamine
EI = Electron Ionization
PCI = Positive Chemical Ionization
NCI = Negative Chemical Ionization
RT = Retention Time
MF = Molecular Formula
MW = Molecular Weight
amu = atomic mass unit
Keywords: Bhilwa, Phytochemical, Herbal medicine, Anacardiaceae
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