EVALUATION OF HEPATOCYTES REGENERATOR POTENTIALITY OF EE-LEF AGAINST CCL4 INDUCED RAT HEPATOCYTES

Mahendar Boddupally*, Monica Chopra, Dr. S. Shoba Rani, Shubhankar Saha

ABSTRACT

The liver is the only visceral organ that possesses remarkable capacity to regenerate. The liver can regenerate after either surgical removal or after chemical injury. It is known that as little as 25% of the original liver mass can regenerate back to its full size. The process of regeneration in mammals is mainly compensatory growth because only the mass of the liver is replaced not the shape. Liver regeneration involves replication of the liver cells, mainly hepatocytes, followed by other cells such as biliary epithelial cells and sinusoidal endothelial cells. Once cell proliferation is completed, the newly divided cells undergo restructuring, angiogenesis and reformation of extracellular matrix to complete the regeneration process. The main objective of present research work is to screen the bio molecules present in EE-LEF and evaluate the in vivo hepatocytes regenerators’ potentiality. etc. The in vivo hepatocytes regenerator’s potentiality was performed against CCl4 induced rat hepatocytes. The results obtained from the present experimental data were indicated that the elevated levels of SGOT, SGPT, ALP and Serum bilirubin due to CCl4 intoxication were reduced significantly in rats, after treatment with EE-LEF. Treatment with EE-LEF at a 500 mg/kg b. w. significantly decreased the SGOT, SGPT, ALP, Serum Bilirubin levels by 26.1%, 47.16%, 24.34% and 43.58% respectively. Silymarin used as standard drug showed a reduction of 55.09%, 68.98%, 57.46% and 60.68% receiving CCl4 alone. So depending upon the present data it was confirmed that the biochemical parameters of the group treated with EE-LEF was significantly lower than the CCl4-treated group.

Keywords: Regeneration, hepatocytes, angiogenesis, endothelial cells, SGPT etc.