SYNTHESIS OF SILVER NANOPARTICLES USING FLAVONOID: APIGENIN AND ITS ANTIBACTERIAL EFFECT
K. Radhakrishnan, T. Rettinaraja*, A. Mohan, S. Syed Jainulabideen
Apigenin, a dietary plant derived flavonoid was isolated from the leaves of Smilax perfoliata. The separation was carried out by different chromatographic techniques and their structures were elucidated by IR spectra, MS, 1HNMR, and 13CNMR spectroscopic methods. Further, the isolated flavonoid – apigenin was used for the synthesis of silver nanoparticles (AgNPs). Aqueous solution of pure flavonoid mixed with 1mM AgNO3 solution were exposed to bright sunlight to prepare the nanoparticles. Characterization of the synthesized nanoparticles by UV–Visible spectrophotometer, X-ray diffraction and scanning electron microscopy revealed that the synthesized silver nanoparticles were 10–80 nm in size and polydispersed in nature. Bactericidal effect against common pathogens of the synthesized silver nanoparticles was investigated. It is concluded that AgNPs synthesized using Apigengin as reducing agent showed higher stability and better antibacterial activity.
Keywords: Apigenin, Flavonoids, Silver Nanoparticle, Bio-synthesis, Smilax perfoliata.
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