IN VITRO CYTOTOXICITY TESTING OF BIOSYNTHESISED SILVER NANOPARTICLE OF ANDREDERA CORDIFOLIA IN PROSTATE CANCER CELLS (PC-3)

Rajathi K.*, N. Kannikaparameswari and S. Suja

ABSTRACT

Silver nanoparticles because of their interesting characteristics are currently the most widely used nanoparticles. One of the interesting properties is that they display antimicrobial activity. Concomitantly they exert cytoprotective effect on human cells. It is of interest to validate and analyse this property. In the present study, silver nanoparticles were synthesised with particle size between 40-60nm in diameter. These were then used to analyse their uptake and consequent cytotoxic effects on Human Prostate cancer cells (PC-3). The formation of Ag-NPs was confirmed by UV-Visible Spectroscopy, X-Ray Diffraction (XRD) pattern, Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The synthesized Ag-NPs were predominately spherical in shape and polydispersed. Fourier Transform Infra-Red (FT-IR) spectroscopy analysis showed that the synthesized nano-Ag was capped with bimolecular compounds which are responsible for reduction of silver ions. The AgNPs showed potent cytotoxic activity against the human prostate cancer (PC-3) cell line at higher concentrations.

Keywords: Cytotoxic effect, PC-3 cell line, silver nanoparticle,Andredera cordifolia,Cell viability,etc.