ESTIMATION OF LAMOTRIGINE IN HUMAN PLASMA BY LCMS/MS
Srinivasa Reddy, Imran Ahmed, Santosh Kumar, Licto Thomas, Arindam Mukhopadhyay* and Saral Thangam
A robust, selective and sensitive LCMS/MS method was developed for quantification of lamotrigine in human plasma. Lamotrigine is an anticonvulsant drug used for seizures. The drug as well as labeled lamotrigene used as an internal standard was extracted from human plasma by liquid – liquid extraction method. Chromatographic separation was performed on a SCX cation exchange column using an isocratic mobile phase (5mM ammonium acetate in 0.05% formic acid: acetonitrile:: 30:70, v/v) at a flow rate of 1.0ml/min. It was detected in LCMS/MS in positive ion mode with electrospray ionization (ESI) interface. The method was validated in the concentration range of 10ng/ml to 8000ng/ml as per FDA guidelines. Mean recovery of lamotrigine was 72%. The assay accuracy was 99.68 – 110.15% of the nominal values. No matrix effect was noticed in this method.
Keywords: Lamotrigine, Cation –exchange column, LCMS/MS, Human Plasma.
[Full Text Article]