SCREENING FOR GENETIC MUTATIONS TO SALT TOLERANCE OF PHASEOLUS VULGARIS L. IN VITRO TREATED WITH SALT STRESS USING ELECTROPHORESIS TECHNIQUE

Hashim K. Mohammed Al-oubaidi* and Rana Ihssan Hamad

ABSTRACT

Sterilized embryos excised from mature seeds of kidney bean (Phaseolus vulgaris L.) were cultured on (MS) medium supplemented with vitamins and growth regulators. Results showed that 2,4-D at (3) mg/l was the best for callus production. Callus was then exposed to different levels of salt mixtures, which were ( NaCl, CaCl2 and MgCl2) in a ratio of 2:2:1 at concentrations of( 0, 50, 100, 150, 200and250) mM added to the culture medium. To produce genetic variation we soaked the callus in SA at concentration of (0.1)mM for (30) min. Callus cultures treated with(0.1) mM of SA for(30) min were cultured on MS medium containing (0 – 250) mM salt mixtures. An experiment of total protein analysis for callus treated with screening and selection or treated with SA in different concentrations was carried out by using electrophoresis to prove any possible differences may be happened in callus tissues which undergo the previous mentioned stresses. Differences were noticed in protein bands: in migration distance, number of bands intensity of appearance and in the molecular weight, and these results were compared with un exposed treatments .

Keywords: Phaseolus vulgaris, In Vitro, salt tolerance, electrophoresis.