EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

SCREENING FOR GENETIC MUTATIONS TO SALT TOLERANCE OF PHASEOLUS VULGARIS L. IN VITRO TREATED WITH SALT STRESS USING ELECTROPHORESIS TECHNIQUE

Hashim K. Mohammed Al-oubaidi* and Rana Ihssan Hamad

ABSTRACT

Sterilized embryos excised from mature seeds of kidney bean (Phaseolus vulgaris L.) were cultured on (MS) medium supplemented with vitamins and growth regulators. Results showed that 2,4-D at (3) mg/l was the best for callus production. Callus was then exposed to different levels of salt mixtures, which were ( NaCl, CaCl2 and MgCl2) in a ratio of 2:2:1 at concentrations of( 0, 50, 100, 150, 200and250) mM added to the culture medium. To produce genetic variation we soaked the callus in SA at concentration of (0.1)mM for (30) min. Callus cultures treated with(0.1) mM of SA for(30) min were cultured on MS medium containing (0 – 250) mM salt mixtures. An experiment of total protein analysis for callus treated with screening and selection or treated with SA in different concentrations was carried out by using electrophoresis to prove any possible differences may be happened in callus tissues which undergo the previous mentioned stresses. Differences were noticed in protein bands: in migration distance, number of bands intensity of appearance and in the molecular weight, and these results were compared with un exposed treatments .

Keywords: Phaseolus vulgaris, In Vitro, salt tolerance, electrophoresis.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing