AUTOANTIBODIES DIABETES AND MICRONUTRIENTS IN TYPE 1 DIABETICS AND SIBLINGS OF ABIDJAN DISTRICT, IVORY COAST.
Chiayé Claire Antoinette Yapo-Crezoit*, Aïssé Florence Judith Trébissou1, Francis Adou Yapo, Sana Khlif, Amina Lahyani, Koumba Agbo-Soumahoro, Fatma MaKni Ayedi, Hatem Masmoudi, Joseph Allico Djaman, Mireille Dosso
Context: the presence of diabetes autoantibodies has been reported as a factor of predisposition of diabetes in siblings of type 1 diabetics. Objective: Search autoantibodies of diabetes anti-ICA, anti-GAD, anti-IA2 in type 1 diabetics and their siblings of district of Abidjan, Ivory Coast, associated with the dosage of micronutrients such as phosphorus, magnesium and calcium. Materials and methods: The study population consisted of 49 people, including 19 type 1 diabetics (T1D) and 30 siblings whose first degree of the blood was taken. T1D were recruited to University Hospital center (U.H.C) of Yopougon and Treichville and two NGOs. Serum obtained allowed the determination of anti-islet cell autoantibodies (anti-ICA) detected by the immunofluorescence method on monkey pancreas, anti-glutamic acid decarboxylase autoantibodies (anti-GAD) and anti- phosphatase IA2 detected by ELISA. The dosage of phosphorus, magnesium and calcium was made by the cobas 6000 Roche/Hitachi. Results: Anti-ICA was positive to 5.26% in T1D and absent in siblings, anti-GAD were positive in the T1D to 57.89% and 3.33% for the siblings. The anti-IA2 was positive in the T1D to 47.36% and 16.66% for siblings. The anti-ICA-GAD-IA2 combination was present in 5.26% of T1D and absent in the siblings. The GAD-IA2 combination was present in 31.57% of T1D and 3.33% among siblings. The average phosphorus in T1D was 1.86±1.06 mmol/L versus 2.39±1.51mmol/L in the siblings; twice higher than normal values. Conclusion: Autoantibodies diabetes in siblings is a marker for the imminence of autoimmune diabetes. Phosphorus is an important marker for the detection and follow up T1D.
Keywords: diabetes autoantibodies, phosphorus, magnesium, calcium, Type 1 diabetes.
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