EVALUATION OF OXIDANT AND ENZYMATIC ANTIOXIDANT STATUS IN ASYMPTOMATIC SUBJECTS WITH HIV-1.

Adeoti Mansour Franck*1, Camara-Cisse Massara, Monteomo Gnate Francois, Koffi Gervais, Kolia Innocent, Djaman Allico Joseph and Dosso Mireille

ABSTRACT

Chronic viral infection with HIV is characterized by progressive and irreversible destruction of CD4 lymphocytes causing oxidative stress and antioxidant reaction. This study aimed to evaluate the immune responses, the balance between oxidant status and antioxidant capacity after in the Acquired Immunodeficiency Syndrome. The authors conducted a prospective study of 60 subjects aged 18 to 65 both sexes, including 30 people with VIH1et 30 healthy control subjects HIV-. In these patients and witnesses, it was made HIV status with an Elisa test and discriminative test (Immunocomb II HIV 1 & 2 BiSpot / PBS Orgenics) and counts as lymphocyte subsets (CD4+ and T-CD8+) is performed by the direct immunofluorescence technique. GPx was assayed by Elisa, SOD and catalase using the PLC Cobas Mira® (Roche Diagnostics) using Randox reagents. There was a significant reduction (p <0.05) in the number of CD4 cells (373.13 ± 69.90 vs 750 ± 30) and CD8 (759.28 ± 131.58 versus 1450 ± 46) patients HIV-1 compared to control subjects. However, a significant increase (p<0.05) markers peroxidation, TBARS (0.99 ± 0.26 versus 3.32 ± 0.40 nmol/MDA) and proteins, OAPP (39, 49 ± 21.20 versus 62.28 ± 13.70 μmol) which reflect an important oxidative stress. The low concentrations significantly (p <0.05) of GPx (40 ± 11 versus 82 ± 15 IU/gHb), SOD (1095 ± 20 vs 1313 ± 41 IU/gHb) and catalase (125 ± 18 versus 210 ± 13 IU/gHb) respectively related to chronic oxidative stress, are more pronounced among the HIV-1 patients. Conclusion: Considering the fall in activity of GPx, SOD and catalase in subjects with HIV-1 infection, they are exposed to oxidative stress and long term consequences.

Keywords: TBARS, OAPP, Superoxide dismutase, Glutathione peroxidase, Catalase, HIV.