ANALYSIS OF LINAGLIPTIN IN TABLET DOSAGE FORM BY UV SPECTROSCOPY METHOD, ITS DERIVATIVES AND DIFFERENCE SPECTRA
R.K. Sangeetha* and T. Subashri
A simple and rapid method of analysis of linagliptin has been established by validated UV spectroscopy method, difference spectroscopy and first order & second order derivative spectra after the identification of drug with its IR spectra interpretation. The UV spectroscopic method was validated according to ICH guidelines for its Linearity, Intraday precision, Interday precision, Accuracy, Limit of Detection and Limit of Quantification. The solvent selected was methanol and the maximum absorbance was found at 228 nm. The linearity range was found between 5-40 mcg/ml with a correlation coefficient of 0.9985 and the slope of the curve was found to be 0.0406. The precision values were found to be below 1 showing its repeatability and reproducibility of the method. The accuracy of method was found by the analysis of formulation and recovery studies at 100% level with % RSD value of 0.5472. The difference spectroscopy method was performed with 0.1 M HCl and 0.1 M NaOH. The difference absorbance was used to calculate the assay value and was found to be 95%. The first order spectra and the second order spectra was obtained from the zero order spectrum of standard drug linear range of 10-40 mcg/ml at the wavelength of 238 nm and 228 nm respectively were selected for the calibration graph. The correlation coefficient was found to be 0.9982 and 1.0 for first order and second order spectra. The method adopted for the analysis of linagliptin are economical, reliable, simple and accurate. This metho can be used for further studies of linagliptin.
Keywords: The method adopted for the analysis of linagliptin are linagliptin.
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