EVALUATION OF ANTIMICROBIAL ACTIVITY OF THE AQUEOUS AND CHLOROFORM EXTRACTS OF LEAVES OF COUROUPITA GUIANENSIS BY WELL DIFFUSION METHOD

Praveen Kumar Uppala*, Dr. K. Atchuta Kumar, D. J. Vinay Ramji and Uma Shankar Gorla

ABSTRACT

Objective: To investigate Antimicrobial activity (Antibacterial, Antifungal) of the Aqueous and Chloroform Extracts of Leaves of Couroupita guianensis by Well Diffusion Method. Methods: The Antimicrobial activity (Antibacterial, Antifungal) of the Aqueous and Chloroform Extracts of Leaves of Couroupita guianensis by Well Diffusion Method and the results were compared for the both extracts. Antibacterial activity is compared with standard antibiotic Penicillin (10mg/ml) and antifungal activity is compared with standard antibiotic Clotrimazole (10mg/ml). Results: The chloroform extract of Couroupita guianensis showed better activity against the fungus like Candida albicans with the zone of 4.36±0.84 followed by Aspergillus niger with zone of diameter 2.3127±0.668 and the aqueous extract shows better activity against the bacteria like, Staphylococcus aureus zone of diameter is 2.2 Escherichia coli the zone of diameter 2.67. In the present study, both in bacteria and fungi chloroform and aqueous extracts showed a varying degree of inhibition of the growth against tested organism. Conclusion: From the above finding concluding that, this study evaluated the inherent antifungal activity of chloroform as well as the antifungal activity of aqueous extract of couroupita guianensis. From the obtained results it can be concluded that although chloroform itself has antifungal activity, chloroform extract of couroupita guianensis has a synergistic activity.

Keywords: Couroupita guianensis, Candida albicans, Aspergillus niger, Staphylococcus aureus, Escherichia coli.