MUCUNA SANJAPPAE SHOWS PROMISING ANTI-PARKINSON’S ACTIVITY BY REDUCING OXIDATIVE STRESS IN MPTP INDUCED MOUSE MODEL
R. R. Patil*, Sachchida Nand Rai*, J. P. Jadhav and Surya Pratap Singh
To evaluate anti-Parkinson‟s activity of ethanolic extract of Mucuna sanjappae in MPTP induced mouse model. In this study, our efforts on the evaluation of neuroprotective efficacy of newly described Mucuna species, M. sanjappae in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced mouse model of Parkinson‟s disease (PD). Twenty Swiss albino mice were randomly divided into five groups: Control, MPTP and three different doses of MS seed ethanolic extract in MPTP induced mice group (50, 100 and 150 mg/kg body weight (wt)). After 21 days, the behavioral studies, neurobiochemical studies and immunohistochemical studies were performed in different groups of animal. There was improvement in behavioral parameters of the MS treated mice with optimum dose of 100 mg/kg body wt. Our results demonstrate generation of oxidative stress in the nigrostriatal region of MPTP exposed mice. There was a marked increase in lipid peroxidation, nitrite level and catalase activity and decrease in glutathione level in Parkinsonian mice whereas; significant restoration was observed due to the MS treatment of 100 mg/kg body wt. The protection of dopaminergic neurons was confirmed by TH immunostaining. MS reduces behavioral impairments, oxidative stress generated due to MPTP and also prevents dopaminergic neurodegeneration thereby showing its efficacy as a future anti-Parkinsonian agent.
Keywords: Parkinson?s disease, Mucuna sanjappae, MPTP, Tyrosinase hydroxylase, Dopamine, Oxidative.
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