PHARMACOLOGICAL EVALUATION OF METHOTREXATE INDUCED HEPATOTOXICITY IN WISTAR RATS
Kumari Shalini and Kumari Savita*
Objective: Drugs and chemicals are considered as one of the major causes for hepatotoxicity, inducing direct damage through multiple pathways including oxidative stress. Amlycure DS is a polyherbal hepatoprotective formulation indicated as liver protective. The present study was aimed to investigate the protective effect of Amlycure DS in methotrexate induced hepatotoxicity in wistar albino rats. Method: 30 rats of either sex were divided into 5 groups. Group-I serves as normal control receiving normal standard pellet diet and water ad libitum. Group-II serves as toxic control with methotrexate (100μg/kg i.p.) once daily for 40 days. Group-III serves as standard control receiving silymarin(63mg/kg) orally, daily for 40 days. Group IV and V received Amlycure DS at doses 1406 and 5608 mg/kg orally respectively for 40 days. At the end of 40 days methotrexate injection induces significant oxidative stress with rise in serum ALT, serum AST, BIT, BID, AST where as levels of serum albumin and serum protein were reduced, in addition Antioxidant glutathione, Catalase value was reduced. Treatment of rats with different doses of Amlycure DS (1406 and 5608 mg/kg) significantly (P<0.05) ameliorated the alteration induced by methotrexate in oxidative stress and serum biochemical analysis. Conclusion: Based on these results, we conclude that Amlycure DS protects liver from oxidative damage induced by methotrexate.
Keywords: polyherbal hepatoprotective, Amlycure DS.
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