CHARACTERIZATION OF BACTERIOCIN PRODUCED BY Bradyrhizobium japonicum ISOLATED FROM MAIZE FIELD

Ambika R*, Senthilkumar G, Panneerselvam A and Sengottaian N

ABSTRACT

Bacteriocin producing Bradyrhizobium japonicum strain was isolated from maize field. Bacteriocin production by Rhizobium sp was carried out in modified TY medium and extracted using 0.22μm membrane filter. The bacteriocin has purified by ammonium sulphate precipitate and SDS-PAGE. Biochemically it was pure protein moiety and the molecular weight was 28kDa. It also showed broad range of antibacterial activity against some major pathogens. The study revealed the possibility of using bacteriocin as a biocontrol agents.

Keywords: Bacteriocin, Bradyrhizobium japonium, SDS-PAGE, antagonistic activity, Well diffusion method.