PHARMACOLOGICAL ASSESSMENT OF DELONIX REGIA FLOWERS FOR HEPATOPROTECTIVE ACTIVITY AGAINST PARACETAMOL-INDUCED LIVER TOXICITY

Abubaker Siddiq*, Moulya M.V, Siraj Ahamed S and Bharathi D.R

ABSTRACT

The objective of this study is to assess hepatoprotective activity of ethanolic extract of Delonix regia flowers (EEDRF) against Paracetamol induced hepatotoxicity in Rats. The toxicity were induced in rats by paracetamol 2g/kg orally, in four groups of rats (two test, standard and toxic control). Two test groups received EEDRF at doses of 250 mg/kg and 500 mg/kg. Standard group received silymarin (100 mg/kg) and toxic control received only Paracetamol. Control group received only vehicle. On 7th Day animals were sacrificed and liver enzymes like Serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), Serum alkaline phosphatase (ALP), Serum total and direct bilirubin were estimated in blood serum and invivo antioxidant enzymes like Tissue Glutathione (GSH), lipidperoxidation(LPO) and superoxide dismutase(SOD) were estimated in tissue homogenate. The ethanolic extract produced significant reduction in SGPT, SGOT, ALP, total and direct bilirubin, LPO levels and significantly increased the depleted GSH and SOD levels as compared with Paracetamol group. This is further confirmed by histopathological studies. The results of the present study reveal that EEDRF has contributed to the reduction of oxidative stress and the protection of liver in experimental animals.

Keywords: Delonix regia, Paracetamol, Silymarin, hepatoprotective, Lipid peroxidation.