EVALUATION OF NEW EPIGENETIC MARKERS SPG20, ITGA4 AND ALX4 IN PLASMA OF COLORECTAL CANCER PATIENTS.
Rezvaneh Foroughi, Somayeh Chavoshei, Hasan Ashoori, Shahla Mohammad Ganji, Ghasem Azizi Tabesh, Susan Farmand-Rad and Mahmood Tavallaei*
Background: Colorectal cancer (CRC) has a high incidence and mortality. Remarkable increase in the incidence of CRC has been reported in different populations in the past decades. The risk is influenced by both environmental and genetic factors. DNA methylation is an effective molecular biomarker for diagnose of the cancer. Materials and Methods: In this study, blood samples were obtained from 73 individuals (41 cases vs. 32 controls) to test the performance of three gene markers, SPG20, ITGA4 and ALX4, using Methylation Specific PCR (MSP) method. Results: Results showed that the promoters of SPG20, ITGA4 and ALX4 genes are hypermethylated in 68.2%, 60.9% and 68.2% of patients respectively. Conclusion: Collectively it has concluded that the detection of simultaneous hypermethylation of SPG20/ITGA4/ALX4 promoter can specifically be applied as a biomarker in the diagnosis of patients with cancer risk and can be promising before colonoscopy.
Keywords: CRC-methylation- ITGA4- SPG20- ALX4.
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