SELECTION OF MACRONUTRIENTS AND VITAMINS FOR VANCOMYCIN PRODUCTION BY AMYCOLATOPSIS ORIENTALIS USING PLACKETT –BURMAN DESIGN

Gopal Reddy*, P. Naga Padma and A. Bhaskar Rao

ABSTRACT

Screening of diverse phosphate, sulphate and vitamins for glycopeptide antibiotic vancomycin production was done using Plackett-Burman statistical design. Different sulphate and phosphate salts of ammonium, sodium and potassium and thiourea numbering 11 were screened using 12 experimental designs. Similarly different vitamins were screened by the same 12 experimental design of Plackett-Burman. The fermentation was carried out for 10 days and the vancomycin yields were subjected to statistical analysis. Significant phosphate, sulphate, and vitamins were short listed based on regression coefficients and t-values. The significant phosphate sources were disodium hydrogen phosphate, potassium dihydrogen phosphate, and sulfate sources were, sodium thiosulphate, ammonium sulphate. These were short listed for further optimization studies using second step of Plackett-Burman screening and Response Surface Methodology (RSM). Optimized production medium is a necessity for any commercial production for significant product yield with minimum inputs. This screening strategy not only short listed the efficient diverse nutrients but also improved the antibiotic yields as the combined effects of the nutrients were also considered.

Keywords: Amycolatopsis orientalis, Vancomycin, phosphate sources, sulphate sources, vitamins, Plackett-Burman.