EVALUATION OF NEPHROPROTECTIVE PROPERTIES OF AQUEOUS AND ETHANOLIC EXTRACTS OF GOMPHRENA CELOSIOIDES, COLA NITIDA AND ENTENDROPHRAGMA ANGOLENSE AGAINST GENTAMICIN INDUCED RENAL DYSFUNCTION IN THE ALBINO RATS.

Bamba Abou*, Yapi Houphouet Felix, Aka Kouadio Ayebe Edwige and Djyh Bernard Nazaire

ABSTRACT

Nephrotoxicity is one of the important side effects of certain antibiotics such as gentamicin. The purpose of this study was to determine the protective effect of aqueous and ethanolic extracts of entandrophragma angolense, cola nitida and gomphrena celosioides on gentamicin induced nephrotoxicity in rats; using biochemical approaches as a guide. The extractions were performed by macerating 100 g of plant dry powder in 2 liters of distilled water (aqueous extraction) or in 2 liters of water / ethanol mixture (30/70 V / V) (ethanol extraction). The nephroprotective properties were evaluated by comparing the activity of the extracts (200 and 500mg / kg) to that of vitamin E (250 mg / kg) against gentamicin-induced renal toxicity by (80 mg / kg) .Technically; gentamicin is administered to the animals one hour after treatment with the extracts for seven days. The results showed that intra-peritoneal injection of gentamicin causes a significant decrease (p <0.05) of total serum rate of protein and a significant raise (p <0.05) in serum rate of creatinine and urea. The results also show a significant increase (p <0.05) in total protein and albumin in the urine of animals. However, changes in biochemical parameters measured were significantly (p <0.05) attenuated in co-treated rats with the extracts. In conclusion, our studies have shown that treatment with the extracts significantly attenueate (p <0.05) levels of serum creatinine, serum urea, serum protein, proteinuria and albuminuria.

Keywords: nephrotoxicity, gentamicin, extraits, Entandrophragma, Cola, gomphrena.