IN-VIVO INVESTIGATION OF ANALGESIC, ANTIPYRETIC ANTI-DIARRHEAL AND ANXIOLYTIC ACTIVITY OF BLUMEA DENSIFLORA DC.

Mohammad Hossain, Md. Mazharul Islam, Md. Abul Kalam Azad, Abdullah Al Faruq, Syed Mohammed Tareq and Md. Qamrul Ahsan*

ABSTRACT

The present study was conducted based on the traditional uses of Blumea densiflora and to evaluate in-vivo analgesic, antipyretic, antidiarrheal and anxiolytic activities. Analgesic effect was assessed using Eddy’s hot plate models and antipyretic activity was determined by Brewer’s yeast-induced pyrexia in mice. The ethanol extract of B. densiflora (400mg/Kg, b.wt.) significantly (P˂0.05) decreased latency time in the hot plate method and it also reduced rectal body temperature in Brewer’s yeast-induced pyrexia at the dose of 500mg/Kg body weight of plant extract. The antidiarrheal effect was studied in mice against castor oil induced diarrhea at the dose of 500mg/Kg body weight. The ethanolic extract of Blumea densiflora (EEBD) reduces the number of the faces. The anxiolytic activity was evaluated by open field test, hole cross test and swing test. The result showed that EEBD (500mg/Kg b.wt.) decreases the number of field cross, hole cross and swing which was statistically significant (P˂0.05). Altogether these results showed that the EEBD may have analgesic, antipyretic, antidiarrheal and anxiolytic activities which provide a scientific evidence for its traditional claim.

Keywords: In vivo, Plant extract, analgesic, antipyretic, antidiarrheal and anxiolytic.