MICROSCOPIC EVALUATION, ISOLATION AND ANTIMICROBIAL ACTIVITY OF PHYTOCONSTITUENTS FROM THE LEAVES OF BLECHNUM ORIENTALE LINN
Dr. Vijay Nigam*, Pradeep Vikram, Manoj Chandwani and Priyanka Jain
Blechnum orientale Linn belongs to family Blechnaceae, found throughout India. The methanol extract of the plant was subjected to column chromatography for the isolation of phytoconstituent. saponins, tannins, terpenoids, flavonoids and alkaloids. Total two constituent has been isolated and characterized as Trimethyl ellagic acid and 11, 12 dimethoxy, 2-rhamnosyl, 4-ol, 5- keto flavone. Their structures were elucidated by spectroscopic methods such as UV, IR,
NMR and LCMS. The antimicrobial activity of the extracts was determined by disc diffusion method (NCCLS, 1997) in petriplates containing NA medium (20 mL media/plate), respectively.
Keywords: Blechnum orientale Linn, Trimethyl ellagic acid, 11, 12 dimethoxy, 2-rhamnosyl, 4-ol, 5- keto flavone, spectroscopic method, disc diffusion method.
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