ANTICONVULSANT ACTIVITY OF EXTRACT AND FRACTIONS OF PINUS ROXBURGII SARG. AGAINST PENTYLENETETRAZOLE INDUCED SEIZURE AND MAXIMAL ELECTROSHOCK (MES) INDUCED SEIZURES IN ALBINO RATS.
Dev Narayan Choudhary*, Waseem Rizvi, Anil Kumar and Ompal Singh
Objectives: The aim of the present study was to evaluate the anticonvulsant potential of extract and fractions of Pinus roxburgii Sarg. against Pentylenetetrazole and maximal electroshock (MES) induced seizures in albino rats. Materials and Methods: The ethanol extract (300 and 600 mg/kg dose) and Chloroform, n-Hexane, n-Butanol and Ethylacetate fraction (250 and 500 mg/kg dose) of Pinus roxburgii Sarg. were tested at two doses (minimum and maximum) for its anticonvulsant activity using pentylenetetrazole (PTZ) induced and maximal electroshock (MES) induced seizures in albino rats. Results: There was significant (P<0.05) reduction in the duration of Tonic hind limb extension (THLE), tonic limb flexion (TLF) and clonic phase observed with extract and fractions but maximum reduction was observed with chloroform and n-Hexane fractions. Against Pentylenetetrazole(PTZ) induced seizure there was significant (P<0.05) reduction in the duration of myoclonic jerks, duration of clonic phase seen in all treated groups but maximum change was observed with 500 mg/kg Chloroform, 500 mg/kg n-Hexane and 600 mg/kg Extract treated group. Conclusion: The results demonstrated that extract and fraction of Pinus roxburgii Sarg. poses anticonvulsant potential against Pentylenetetrazole induced seizure and maximal electroshock (MES) induced seizures.
Keywords: Pentylenetetrazole induced seizure, maximal electroshock (MES) induced seizure, Pinus Roxburgii Sarg., extract, fractions
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