EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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Abstract

ARISTOLOCHIA BRACTEOLATE: POTENT ENHANCER OF B CELLS AND INHIBITS T CELL POPULATION AGAINST SPECIFIC PROTEIN ANTIGEN

Amit Gupta*, Jayaprakash and Bharat Shinde

ABSTRACT

Objective- To investigate its immunopharmacological activity of aqueous leaves extract of Aristolochia bracteolate using phosphate buffered saline (PBS, pH 7.2) against hepatitis B vaccine containing surface antigen (HBsAg; 20 μg/ml) and weak antigen i.e. ovalbumin (OVA) pertaining to antibody production determined through Elisa and scrutinize its proliferative response in infected lysed human whole blood with respect to HBsAg and OVA. Methods- For these studies, phytochemical (qualitative) analysis were determined and evaluate the presence of primary (protein) and secondary metabolites in aqueous leaves extract. In addition, indirect Elisa was performed using HBsAg and OVA as coating antigen using variable doses (0.156 – 5 mg) of aqueous leaves extract of Aristolochia bracteolate. In continuation of these immunological studies, antigen specific immune response were determined in infected human whole blood using HBsAg (20 μg/ml, 50 μl) and OVA (1 mg/ml; 50 μl) using MTT assay. Results- The results showed that aqueous extract showed qualitatively the presence of secondary metabolites including protein (40 KDa and 100 KDa) content which is determined through SDS (sodium dodecyl sulphate) PAGE (polyacrylamide gel electrophoresis). In addition, this aqueous leaves extract showed enhancement in anti-HBsAg and anti-OVA IgG titre as compared to standard and control but there is sudden decline in proliferation containing HBsAg and OVA at higher doses. Conclusion- Our data suggest that aqueous leaves extract of this medicinal plant may help to raise antibodies against HBsAg and OVA but sudden decline in HBsAg and OVA proliferative response in infected lysed human whole blood. In other words, Aristolochia bracteolate could be a potent immune enhancer of B cells and inhibitor of T cells.

Keywords: Aristolochia bracteolate; hepatitis B vaccine; ovalbumin; splenocyte; Elisa.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia