IN-VITRO EVALUATION OF CO-ADMINISTRATION OF CHLOROFORM LEAF EXTRACT OF CHROMOLAENA ODORATA ON THE ANTIMICROBIAL ACTIVITY OF CLINDAMYCIN AND ITRACONAZOLE.
Sylvester Chika Ohadoma*
Objective: To investigate the interacting effects of co-administration of leaf extract of Chromolaena odorata on the antimicrobial activity of clindamycin as well as itraconazole, in-vitro. Materials and Methods: The chloroform extract of the dried leaves obtained by 48 h cold maceration was evaluated using agar cup diffusion technique; and checkerboard technique for evaluation of interaction between the two antimicrobial agents. Results: The extract showed inhibitory effect against Staphylococcus aureus and Aspergillus niger at concentrations of 5 mg/ml and 10 mg/ml respectively. Clindamycin had MIC of 0.003 mg/ml against Staphylococcus aureus while itraconazole had MIC of 0.0002 mg/ml against Aspergillus niger. Concomitant administration of extract and drugs revealed that some ratios showed additive properties, some other ratios were synergistic, while some showed indifferent activities against both isolates. Conclusion: Clindamycin and itraconazole exhibited synergistic interaction with C. odorata leaf extract against microbial infections. At a given ratio, the combination of Chromolaena odorata and clindamycin or itraconazole could be of possible clinical significance in the treatment of bacterial and fungal infections.
Keywords: Chromolaena odorata, clindamycin, itraconazole, co-administration, in-vitro.
[Full Text Article]