INVESTIGATION ON HEPATOPROTECTIVE ACTIVITY OF PSIDIUM GUAJAVA AGAINST RIFAMPICIN AND ISONIZID INDUCED ???????OXICITY IN RAT

Astha Gupta*, Ashish Kumar, Vishal Dubey

ABSTRACT

Background: The main aim of this project was to evaluate the hepatoprotective property of aqueous extract of Psidium guajava leaf against rifampicin and isoniazid induced hepatotoxicity. The combination of Anti-tuberculosis drugs isoniazid and rifampicin have property to treat tuberculosis, these drugs are used are associated with toxic reaction in tissues, mainly in the hepatic region, leading to liver disease (hepatitis). In regenerating and protecting the liver, Guava, an aqueous plant Leaf extract derived from the Guava plant, is effective. Methods: Wistar albino rats weighing 150–200 g were used to form 5 study groups, each group consisting of 6 rats. Isoniazid and Rifampicin (50 mg/kg each) body weight; was used to induce hepatotoxicity and Guava Leaf as a standard. Hepatoprotective activity of toxicity was evaluated by monitoring the serum enzyme levels and histopathology. Results: As evidenced by biochemical measurements, Isoniazid and Rifampicin treatment induced hepatotoxicity in rats: AST (aspartate aminotransferase), ALP (alkaline phosphatase) and ALT (serum alanine aminotransferase) activities and when drugs were administered to animals, their total bilirubin levels increased, and their albumin and total protein levels decreased. The livers of animals treated with drugs also showed changes in histopathology. Drugs induced significant biochemical and histological changes when AAM was administered simultaneously. Conclusion: AAM exhibited hepatoprotective action against antitubercular drugs induced hepatotoxicity.

Keywords: Hepatoprotective, Psidium guajava, Rifampicin, Isoniazid.