N-ACETYLCYSTEINE PREVENTS CORD DERIVED STEM CELLS FROM H2O2 INDUCED INJURY IN VITRO

Dr. Fatima Ali*, Hadia Rafique and Nadia Wajid

ABSTRACT

Stem cell therapy is a promising approach for repair of tissue injury. However, the efficiency of transplanted stem cells is greatly hampered by the injury conditions in the body. The purpose of present study was to investigate the potential role of an antioxidant N-acetylcysteine (NAC) in in-vitro injury conditions on Whartonʼs Jelly (WJ) derived Mesenchymal stem cells (WJ-MSCs). Injury was inflicted in WJMSCs by treating with 100 μM H2O2 and 1 mM NAC was employed to WJ-MSCs during this injury condition. Cell viability, death and proliferation were assessed by trypan blue exclusion assay, MTT assay and LDH release respectively. Oxidative stress was evaluated by levels of glutathione (GSH), malodialdehyde (MDA) as well as by activity of superoxide dismutase (SOD) and catalase (CAT). 100μM/L H2O2 inflicted injury to WJ-MSCs while 1 mM NAC reduced this injury significantly. Results demonstrated that NAC increased cell viability and proliferation, and decreased cell death. NAC also enhanced antioxidant capacity of WJ-MSCs against H2O2 induced injury. NAC could be a promising therapeutic approach for improving survival of WJ-MSCs under H2O2 induced injury.

Keywords: N-acetylcysteine, Wharton?s Jelly derived Mesenchymal stem cells, Oxidative stress.