N-ACETYLCYSTEINE PREVENTS CORD DERIVED STEM CELLS FROM H2O2 INDUCED INJURY IN VITRO
Dr. Fatima Ali*, Hadia Rafique and Nadia Wajid
Stem cell therapy is a promising approach for repair of tissue injury.
However, the efficiency of transplanted stem cells is greatly hampered
by the injury conditions in the body. The purpose of present study was
to investigate the potential role of an antioxidant N-acetylcysteine
(NAC) in in-vitro injury conditions on Whartonʼs Jelly (WJ) derived
Mesenchymal stem cells (WJ-MSCs). Injury was inflicted in WJMSCs
by treating with 100 μM H2O2 and 1 mM NAC was employed
to WJ-MSCs during this injury condition. Cell viability, death and
proliferation were assessed by trypan blue exclusion assay, MTT assay
and LDH release respectively. Oxidative stress was evaluated by levels
of glutathione (GSH), malodialdehyde (MDA) as well as by activity of superoxide dismutase
(SOD) and catalase (CAT). 100μM/L H2O2 inflicted injury to WJ-MSCs while 1 mM NAC
reduced this injury significantly. Results demonstrated that NAC increased cell viability and
proliferation, and decreased cell death. NAC also enhanced antioxidant capacity of WJ-MSCs
against H2O2 induced injury. NAC could be a promising therapeutic approach for improving
survival of WJ-MSCs under H2O2 induced injury.
Keywords: N-acetylcysteine, Wharton?s Jelly derived Mesenchymal stem cells, Oxidative stress.
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