EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

N-ACETYLCYSTEINE PREVENTS CORD DERIVED STEM CELLS FROM H2O2 INDUCED INJURY IN VITRO

Dr. Fatima Ali*, Hadia Rafique and Nadia Wajid

ABSTRACT

Stem cell therapy is a promising approach for repair of tissue injury. However, the efficiency of transplanted stem cells is greatly hampered by the injury conditions in the body. The purpose of present study was to investigate the potential role of an antioxidant N-acetylcysteine (NAC) in in-vitro injury conditions on Whartonʼs Jelly (WJ) derived Mesenchymal stem cells (WJ-MSCs). Injury was inflicted in WJMSCs by treating with 100 μM H2O2 and 1 mM NAC was employed to WJ-MSCs during this injury condition. Cell viability, death and proliferation were assessed by trypan blue exclusion assay, MTT assay and LDH release respectively. Oxidative stress was evaluated by levels of glutathione (GSH), malodialdehyde (MDA) as well as by activity of superoxide dismutase (SOD) and catalase (CAT). 100μM/L H2O2 inflicted injury to WJ-MSCs while 1 mM NAC reduced this injury significantly. Results demonstrated that NAC increased cell viability and proliferation, and decreased cell death. NAC also enhanced antioxidant capacity of WJ-MSCs against H2O2 induced injury. NAC could be a promising therapeutic approach for improving survival of WJ-MSCs under H2O2 induced injury.

Keywords: N-acetylcysteine, Wharton?s Jelly derived Mesenchymal stem cells, Oxidative stress.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )