ANALYTICAL METHODS FOR SIMULTANEOUS DETERMINATION OF ATORVASTATIN AND RAMIPRIL IN PHARMACEUTICAL FORMULATIONS AND BIOLOGICAL MATRICES: A COMPREHENSIVE REVIEW

Abinesh Sivakumar*, Dr. P. Aravanan

ABSTRACT

Atorvastatin and ramipril are commonly used together to manage cardiovascular conditions, including high blood pressure, abnormal lipid levels, and coronary artery diseases. Accurate and reliable analytical methods are necessary for the simultaneous detection of these compounds in pharmaceutical formulations and biological samples because of their combined therapeutic effects. This review examines different analytical methods created for the measurement of atorvastatin and ramipril, both separately and together. The techniques mentioned are UV spectrophotometry, High-Performance Liquid Chromatography (HPLC), High-Performance Thin Layer Chromatography (HPTLC), and Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). UV spectrophotometric methods are simple and affordable, while HPLC and RP-HPLC methods offer greater sensitivity, specificity, and consistency. HPTLC is an effective option because it offers quick analysis and uses less solvent. The review also covers bioanalytical methods that use reverse phase high-performance liquid chromatography (RP-HPLC) for plasma samples, emphasizing their role in pharmacokinetic and bioavailability research. The findings from different studies suggest that these methods are dependable and appropriate for regular quality control and research purposes. This review offers a detailed summary of the analytical techniques that are currently available and highlights the significance of method validation in achieving accurate and consistent results for the simultaneous measurement of atorvastatin and ramipril.

Keywords: Atorvastatin, Ramipril, HPLC, HPTLC, RP- HPLC, UV Spectrophotometry.