SYNTHESIS CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF PYRAZOLO[3,4-D]THIAZOLE DERIVATIVES OF 1,2,4-TRIAZOLE

Ravi Kumar Saini, Dr. Umesh Kumar*

ABSTRACT

A series of five novel Schiff base derivatives of 4-amino-1,2,4-triazole [3a–e] were synthesized by the condensation of 4-amino-1,2,4-triazole with various 5-(4-substituted phenyl)furan-2-carbaldehydes. The synthesized compounds were obtained in good yields (75–79%) and characterized by elemental analysis, FT-IR, ¹H NMR, ¹³C NMR, and LC-MS spectral techniques. The spectral data confirmed the formation of the azomethine (C=N) linkage and the proposed structures. All the Schiff bases were evaluated for their in vitro antibacterial activity against two Gram-positive (Bacillus megaterium and Staphylococcus aureus) and two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacterial strains using the agar cup diffusion method at 1000 ppm concentration. The results revealed that all compounds exhibited moderate to good antibacterial activity, with the chloro-substituted derivative 3b showing the highest potency against all tested strains. The present study demonstrates that triazole-furan hybrid Schiff bases represent promising candidates for the development of new antimicrobial agents.

Keywords: Schiff bases, 1,2,4-triazole, furan derivatives, antimicrobial activity, antibacterial agents.