ANTI CANDIDAL ACTIVITY OF GREEN TEA (CAMELLIA SINENSIS) EXTRACT AGAINST MDR CANDIDA SP. ISOLATED FROM SOLID WASTE OF LOCAL MARKET

Smaranya Singha Roy, Aniruddha Mukherjee, Dr. Anirban Mukherjee*

ABSTRACT

The increase of the irrational use of antifungal drug has been a major cause of the development of multidrugresistant
(MDR) Candida species, which represents a threat to the limited activity of conventional antifungals. The
research article examines the possibility of using green tea extract as a natural phytochemical counter-measure of
MDR Candida in planktonic and sessile form. In the present research, the selective agar was used to isolate
Candida sp from solid waste disposal of local market and analyze its MDR through resistance profiling. The crude
extract of Camellia sinensis (used as green tea) was applied in this experiment and are characterized using Thin
Layer Chromatography (TLC). To check the efficiency, different drug screening assays were conducted, such as
cup plate assay, MTT assay, MIC assay and the anti-biofilm assay. We have found that green tea bioactive
compounds and more so catechins have an important role in disrupting the biofilm structure of fungi. Overall, this
study recommends the application of the green tea extract as an effective, natural, and readily available intervention
on the management of MDR Candida infection. We provide a new and efficient alternative, which can be used in
clinical practice as a complementary or an alternative of the current antifungal treatments using the inherent
antifungal properties of green tea.

Keywords: Green tea extract; MDR Candida biofilms; Phytochemicals; Anti-biofilm; Antifungal Resistance.