ESTIMATION AND EVALUATION OF LAWSONE IN DIFFERENT HENNA SAMPLES BY USING VARIOUS ANALYTICAL TECHNIQUES

Laya M., Sridevi P.*

ABSTRACT

Lawsonia inermis, often known as henna, is a natural dye plant that is widely used for cosmetic and medicinal purposes. Its main active component, lawsone (2-hydroxy-1,4-naphoquinone), is what gives henna its characteristic reddish-brown colour and therapeutic properties. In order to determine and assess the amount of Lawsone in both natural and commercial henna samples, the current study used UV-visible spectroscopy, HPLC, atomic absorption spectroscopy, and infrared spectroscopy. Cold maceration and sonication were used to create methanolic extracts for efficient Lawsone recovery. UV-visible spectroscopy was utilized to get λ max and initial quantification. Additionally, an HPLC method was created in compliance with ICH guidelines. The use of Atomic Absorption Spectroscopy was used to assess sample safety and heavy metal detection. The developed analytical techniques were shown to be suitable for henna and were straightforward, accurate, precise, and reliable.

Keywords: Lawsone, Henna, UV-Visible Spectroscopy, IR, HPLC, Atomic Absorption Spectroscopy.