INTEGRATING ANALYTICAL METHOD DEVELOPMENT, SELECTION AND VALIDATION IN NDDS

Lavannya Fating*, Lalit Wange, Krutika Burange, Kritika Meshram, Krunal Takarkhede

ABSTRACT

UV-Visible Spectroscopy is a widely used analytical technique that measures the absorption of ultraviolet and light by chemical substances to determine their concentration and structural properties. It operates by passing light through a sample and recording the absorbance at specific wavelengths. The major components of a UV spectrophotometer include a light source, monochromator, sample cuvette, detector, and display system. The technique is fast, nondestructive, and suitable for routine quantitative and qualitative analysis in pharmaceutical research. The present study focuses on the development and validation of a simple, accurate, and precise UV–Visible spectrophotometric method for the estimation of Samples (Metronidazole). UV spectroscopy is based on the absorption of ultraviolet light (200–400 nm) by molecules, causing electronic transitions from lower to higher energy states. The analysis was carried out using a SHIMADZU UV–Visible double beam spectrophotometer (Model UV– 1900I) with quartz cuvettes. The standard solution of Metronidazole was prepared, and serial dilutions ranging from 0.5 to 2.0 µg/mL were analyzed. The wavelength of maximum absorbance (λ max) was observed at 321 nm. The calibration curve plotted between absorbance and concentration showed a linear relationship in accordance with Beer–Lambert’s law, with absorbance increasing proportionally to concentration. The developed method was found to be accurate, reproducible, sensitive, and economical, making it suitable for the routine quality control and quantitative analysis of Metronidazole in bulk and pharmaceutical formulations.[1-2]

Keywords: UV Spectroscopy, Beer-Lambert' Law, Electronic Transition, Metronidazole.