INDUCED CHANGES IN ELECTROPHORETIC PATTERNS OF ESTERASE’S IN INTESTINE AND BRAIN TISSUES OF FRESH WATER CAT FISH. H. FOSSILIS (BLOCH) DUE TO EXPOSURE TO METHYL PARATHION (AN ORGANOPHOSPHATE)

Dr. T. Bheem Rao*

ABSTRACT

The present investigation was aimed at determining the effect of Methyl Parathion (an Organophosphate) on esterase isozyme banding patterns in different tissues such as Liver, Intestine and Brain of fresh water cat fish Heteropneustes fossilis (Bloch) at different time intervals i.e. 24, 48, 72 and 96hrs and was compared with control. The esterase isozymes were quantitatively analyzed by using 7.5٪ native polyacrylamide gel electrophoresis (PAGE) stained with α-naphthyl acetate as substrate. Three different esterase bands were detected and named as Est-1; Est-2 and Est-3 with different relative mobilities such as 0.60; 0.40; 0.30 in liver tissue and named as Est-1; Est-2, Est-3 with different relative mobilities such as 0.60; 0.40; 0.30and 0.15 in intestine tissue and 0.60; 0.40; 0.30 in brain tissue and four different esterase bands were detected All the three esterase bands were found in liver and brain tissues. Among the three esterases Est-1 at 24hrs and Est-2 at 72 hrs and 96hrs in liver tissue, Est-2 at 24hrs, 72hrs and 96hrs Est-4 at 72hrs in intestine are found to be more abundant with highest intensity. The intensity of Est-2 Est-3 was faintly stained in brain tissue, Est-3 in liver tissue Est-4 in intestine tissues were faintly stained. The results revealed that, there are various altrations due to methyl parathion exposure in esterases patterns of the tissues such as brain, intestine, liver of H. fossilis which showed homology in protein bands with minor variations.

Keywords: Esterase, isozymes, PAGE, H.fossilis, Methyl Parathion.