EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

EFFECT OF LIGHT INTENSITY ON SOME SECONDARY METABOLITES OF ARTEMISIA ABROTANUM L. BY TISSUE CULTURE TECHNIQUE.

Yassien Mohamed Yassin, Mohamed Mahmoud ELzefzafy, Gouda T. M. Dawoud*, Heba Shahin and Safaa M. E. Abd El-hameid

ABSTRACT

The effect of light intensity on plant growth of Artemisia abrotanum L. was investigated in vitro. Plantlets of Artemisia abrotanum L. with leaves of 1 cm. long were cultured on suitable medium under different light intensities of 300, 600, 1500, 3000, and 6000 lux, the environment for all growth in vitro was maintained at 25° ± 2°C, with a 16-h photoperiod. The number of shoots, roots, length of shoots and roots in 21 days of culture of plantlets, the collected data were analyzed by one way ANOVA followed by Tuky’s HSD test values represent the mean ±SD of eight replicates and all experiments were repeated three times, mean difference is significant at the 0.05 level, the best result were found at 3000 lux and then 1500 lux on the other hand the least in vitro growth were in 300 lux. Preliminary phytochemical screening of in vivo and in vitro for Artemisia abrotanum L. showed that both samples were contains volatile oil, Carbohydrates and/or glycosides , tannins, flavonoids, sterols and/or triterpenes, resins, while the in vivo sample contain traces of Alkaloid. But both samples free from Saponins, Iridoids, Cardiac glycosides and Anthraquinone glycosides. The total polyphenols and flavonoids were investigated from obtained results in vivo plant sample showed the highest content of polyphenols (271.6mg/100g.d.w) and flavonoids (84.63 mg/100g.d.w) while the lowest content was recorded with the sample extract in 300 lux light intensity condition was (54.90 mg/100g.d.w) and (21.33 mg/100g.d.w).

Keywords: Artemisia abrotanum L., Light intensity. Phenolic compounds, Flavonoids and Secondary metabolites.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )