EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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Abstract

IN VITRO EVALUATION ANTIMICROBIAL ACTIVITIES OF TWO MEDICINAL PLANT EXTRACTS AND COMPARED WITH SOME ANTIBIOTICS

Sukaina R. Neamah*, Maytham T. Qasim, Lamees M. Al-Janabi and Amany Sh. Jaber

ABSTRACT

The Antimicrobial properties of the ethanolic extract of three Medicinal plants, Zingiber officinale rhizomes and Curcuma longa rhizomes were assayed for the in vitro antimicrobial activity against gram-positive standard bacteria represented by Staphylococus aureus and Staphylococcus epidermides and gram-negative standard bacteria represented by Escherichia coli and Pseudomonas aeruginosa using holeplate diffusion method. All the extracts studied in the present investigation exhibited varying degree of inhibitory effect against all the tested human pathogenic bacteria. The results shows the higher inhibition zone of all extracts for gram positive which ranged between 20.94 mm to 18.83 mm compare with gram negative bacteria which ranged between 17.55 mm to 14.38 mm. Also It can be seen antibacterial activity of Zingiber officinale extracts give higher inhibition zones 38.49 mm against all human pathogenic bacteria while the lower inhibition zones of Curcuma longa was 33.22 mm. Compare with The screening results of the Sensitivity test which revealed that The zone of inhibition was recorded higher sensitive to Norfloxacin was 38 mm against S. epidermidis while Erythromycin, Ampicillin and Lincomycin had no effect on it .On the other hand Amoxicillin/ clavulanic acid, Cefdinir, Ceftazidime, Carbeniciln and Cefuroxime had no effect aganst P. aeruginosa, as well as E.coli was resistant against Ceftazidime, Carbeniciln and Cefuroxime. Sulfisoxazole and Ampicilin had no effect on S. aureus. The screening results of the medicinal plants extracts in the present study confirmed a source of antimicrobial agents by the highest sensitivity was recorded of its.

Keywords: In vitro, Antimicrobial, Plant extracts, Antibiotic.


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