EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

ASSESSMENT OF ACUTE TOXICITY AND CYTOTOXICITY OF AQUEOUS EXTRACT (EA), ETHYL ACETATE FRACTION (FA) AND METHANOL FRACTION (FM) OF EUCALYPTUS CITRIODORA.

*Oungbé Monkoué Désiré, D’Almeida Marie-anne, Boga Gogo Lucien, Kouassi Konan, Djaman Alico Joseph and N’guessan Jean David

ABSTRACT

The objective of this study was to investigate the toxicity of E. citriodora. In order to evaluate the toxicity of E. citriodora, the acute toxicity of the aqueous extract, ethyl acetate fraction and methanol fraction of this plant on female rats and cytotoxicity test on NIT-1 cell line were investigated. A fixed large dose of 2000 mg/kg b.w. of aqueous extract, ethyl acetate and methanol fractions was administrated by a single oral gavage according to the OECD procedure. In 2 weeks, aqueous extract, ethyl acetate and methanol fractions did not significantly affect the weight gain, hematological and biochemical parameters of rats treated groups compared with the control group. Cytotoxicity effect of aqueous extract, ethyl acetate fraction and methanol fraction was estimated through a MTT assay. Cytotoxicity tests on NIT-1 cell line disclosed that concentrations of 10, 100 1000 μg/mL inhibited the growth of cells. The concentration inducing cell growth inhibition by about 50% (IC50) were 100 μg/mL. The results of cytotoxicity show toxic effect of E. citriodora extract and fractions on NIT-1 cells. While this work did not showed acute toxicity effect on NIT-1 cells.

Keywords: Eucalyptus citriodora; aqueous extract; ethyl acetate fraction, methanol fraction; cytotoxicity; oral acute toxicity; MTT assay.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )