PHYTOCHEMICAL SCREENING, PROXIMATE ANALYSIS AND ANTIMICROBIAL ACTIVITIES OF DICHROSTACHYS CINEREA (L)

Fatehalrahman F. Magbool* and Mahmoud A. Ibrahim

ABSTRACT

Medicinal plants and their bioactive molecules are always in demand and are a central point of research. To date, herbs have remained useful not only as remedy for different diseases that affect humans and animals, but also as good starting points for the discovery of bioactive molecules for drug development. Therapeutic efficacy of medicinal plants depends upon the quality and quantity of chemical constituents. Preliminary phytochemical screening and antimicrobial activity of Dischrastachys cinerea was carried out to assess the chemical contents, biological and antimicrobial activity. The extraction was carry out according to protocol of WHO 1998 successive extraction method. The phytochemical screening was carry out to show the present of Tannin, Saponins, Alkaloid, Flavonoids, Steroids and sugar. proximate analysis was done to show the present of Moisture 2.270, Ash 9.823, Protein 20.738, Fat 0.806, Fiber 60.390, and carbohydrate 5.973. And antimicrobial was tested against four stander bacteria species: Gram positive bacteria staphylococcus aureus (ATCC 25923)20.44 and Bacillus subtilis (NCTC8236) 18.2 Gram Negative bacteria Escherichia coli (ATCC 25922) 25.50 and pseudomonas aeruginosa (ATCC 27853)21.28 and one stander fungal strain VIZ, Candida albicans (ATCC 7596) 19.94 using disc diffusion method. The study illustrate that, all the extracts of various D. cinerea plant exhibited antibacterial and anti-candidal activities. All the extract were effective against all microorganism that use .Of the two extraction solvent methanol extracts gave better inhibition zones as compared to water extract.

Keywords: Medicinal plants, Bioactive molecules, Phytochemical screening, Antimicrobial activity, Extraction, Bioactive molecules.