EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

A STUDY ON THE BIOFILM FORMATION BY MRSA ON INDWELLING CATHETERS AND IT’S PHYTO-PROPHYLACTICS

G. Krishnakumari* and M. Thangavel

ABSTRACT

Microbial biofilms are extremely complex, consisting of microorganisms attached to a surface and embedded in an organic polymer matrix of microbial origin. The self- produced matrix of extracellular polymeric substances which is also referred to slime, is a polymeric conglomeration generally composed of extracellular biopolymers in various structural forms. Extracellular polymeric substances (EPS) are responsible for the adhesion of microbes and among various EPS producing organisms methicillin resistant strains of Staphylococcus aureus was isolated and identified from on or within indwelling catheters. For the assay of biofilm formation Congo red agar method, Tissue culture plate method and Tube method were performed and obtain results as adherent Staphylococcal cells formed biofilms on all side walls and were uniformly stained with crystal violet in TCP method .The antibacterial activity of various plant leaf extracts like tulsi (Ocimum sanctum), papaya plant, neem and guava(Psidium guajava) were studied and among them neem and tulsi has antimicrobial activity at various concentration. Different materials were selected for the study of biofilm formation on different surfaces and materials like copper (7 x 105CFU/ml), plastic (15 x 105CFU/ml), poly acrylic(15 x 105 CFU/ml) wood and stainless steel(30 x 105CFU/ml) were selected and studied using Tryptone water broth and TSB glu(1%) BHI suc (2%) medium.

Keywords: Biofilms, Methicillin Resistance Staphylococcus aureus, EPS, antimicrobial activity.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia