EFFECT OF DILUENT ON PALM FRUIT WATER AND EGG YOLK STORED AT 3-50OC ON SPERMATOZOA QUALITY OF LOCAL MALE CHICKENS

Nolasco Da Costa*

ABSTRACT

The research aimed to examine the effect of dilution based on palm fruit water and egg yolk with NaCL fisiology on motility, viability and abnormality of spermatozoa membrane of rooster local during cooling process. The randomized Block Design with 5 replications from 4 ejaculations of rooster lokal was applied in this study. Treatments applied were (P0) = 100 % NaCl Fisiologis (P1) = 80 % NaCL Fisiologis + 10 % Egg yolk + 10% Lontar Water, (P2) = 80 % NaCL Fisiologis + 8 % Egg yolk + 12% Lontar Water, (P3) = 80 % NaCL Fisiologis + 6 % Egg yolk + 14 % Palm fruit water Observations on individual motility, viability and integrity of spermatozoa membrane were directly carried out after dilution each day up to the four day and after dilution and kept cooled. The study showed that dilution based on NaCL + egg yolk could mantain the semen quality included motility, viability and integrity of rooster spermatozoa membrane during cooling process. Spermatozoa of rooster diluted with NaCL + 20% egg yolk at the fore day of storage had a progressive motility (45.75%), viability (49.25%) and membrane abnormality (42.70%).

Keywords: lontar water, egg yolk, motility, membrane abnormality, viability sperm.