PHYTO-THERAPEUTIC BALM FORMULATION OF OCIMUM SANCTUM, CALOTROPIS GIGANTEA, AND CINNAMOMUM ZEYLANICUM: EVALUATION AND HPLC PROFILING OF BIOACTIVE EXTRACTS

Namira Shaikh*, Ahilya Sika, Altaf Khan, Prajakta Katekar, Feby noble

ABSTRACT

Inflammation represents a pathological condition that can potentially contribute to various chronic diseases. This study assesses the anti-inflammatory properties of Calotropis gigantea flowers, Ocimum sanctum (Tulsi) leaves, and Cinnamomum verum (Cinnamon) bark. The phytochemical profile of these plant materials was examined, and they were subsequently incorporated into a formulation for a herbal balm. The increasing demand for herbal medicine can be attributed to its high efficacy, affordability, ease of use, compatibility with the human body, and reduced side effects. Rheumatoid arthritis (RA) and gout are common forms of inflammatory arthritis, characterized by chronic pain, joint inflammation, and substantial detriment to quality of life. Although conventional treatments, such as non-steroidal anti-inflammatory drugs (NSAIDs) and corticosteroids, may provide symptomatic relief, they frequently entail adverse effects. This investigation explores a novel herbal formulation to mitigate inflammation and pain associated with RA and gout, employing a synergistic combination of Calotropis gigantea flowers, Ocimum sanctum leaves, and Cinnamomum verum bark. The plant materials underwent methanolic extraction utilizing a Soxhlet apparatus, and qualitative phytochemical analyses confirmed the presence of flavonoids. High-Performance Liquid Chromatography (HPLC) was employed to identify bioactive compounds, including Quercetin, Kaempferol, and Apigenin, which are recognized for their significant anti-inflammatory properties. Further in vitro studies are essential to validate the therapeutic efficacy of this novel herbal preparation.

Keywords: Rheumatoid Arthritis, Gout, Calotropis gigantea, Ocimum sanctum, Cinnamomum verum, Anti-inflammatory, HPLC Analysis, Balm.