PRELIMINARY PHYTOCHEMICAL STUDY, IN VITRO ANTI-INFLAMMATORY AND ANTI-DIABETIC ACTIVITY OF DIFFERENT EXTRACTS OF CISSUS QUADRANGULARIS LINN

Aparna P.*, Rakesh Kumar Jat and Subash Chandran M. P.

ABSTRACT

The present study was aimed to evaluate the phytochemistry, anti-inflammatory and anti-diabetic activity of different extracts of aerial parts of Cissus quadrangularis plant. Initially, the plant material was collected authenticated and dried for powdering. The dried material was powdered in mechanical grinder and the coarse powder thus obtained was extracted in soxhlet apparatus with the solvents of increasing polarity viz., petroleum ether, chloroform, ethyl acetate and methanol. The dried extracts thus obtained were subjected to preliminary phytochemical evaluation. Anti-inflammatory activity of all the extracts was evaluated in vitro by different approaches viz., inhibition of protein denaturation, proteinase inhibitory activity. Anti-diabetic activity was evaluated in vitro by inhibition of α-amylase and α-glucosidase activity. In the preliminary phytochemical evaluation, presence of alkaloids, phenolic compounds, flavanones and flavonoids, carbohydrates, proteins, terpenoids, sterols, saponins and gum and mucilage were found in the tested extracts. The methanol and ethyl acetate extracts showed a significant presence of majority of phytochemicals comparing with other two, the petroleum ether, chloroform extracts. The methanol extract showed significant activity in the in vitro evaluation of anti-inflammatory and anti-diabetic activity. These results are useful for further investigation in the future.

Keywords: Cissus quadrangularis, Preliminary phytochemical evaluation, In vitro anti-inflammatory activity, In vitro anti-diabetic activity.